Length of CytB gene is 1140 bp and has some Cytochrome b is commonly used as a region of mitochondrial DNA for determining phylogenetic relationships between organisms, due to its sequence variability. Forensic Science International, 122 (1). pp. Levels of sequence variation in mitochondrial cytochrome-b gene were examined to ascertain if this molecule can provide a reference point in making decisions concerning species-level distinctions.DNA-sequence data from 4 genera of rodents (Neotoma, Reithrodontomys, Peromyscus, and Sigmodon) and 7 genera of bats (Artibeus, Carollia, Chiroderma, Dermanura, … Material and Methods Eight polymorphic partial cytochrome b sequences The development of the Polymerase Chain Reaction and primers are an integral part of the modern DNA sequencing process. 3. Comparative studies involving cytochrome b have resulted in new classification schemes and have been used to assign newly described species to a … Parasitol Res. We sequenced a 396-bp region of the mitochondrial cytochrome b gene of the most common clinically important Candida species: Candida albicans, C. glabrata, C. parapsilosis, C. tropicalis, C. krusei, … developed for the identification of nucleotide polymorphisms in the Cyt b gene capable of differentiating biological samples derived from the paca from those of domestic species commonly used for the production of meat in the Neotropics (cow, goat, sheep, and pig). Interspecies-specific DNA sequence polymorphisms in the mitochondrial cytochrome b gene … Water Res. We use cookies to help provide and enhance our service and tailor content and ads. mammals, birds, reptiles, amphibians and fishes). Cytochrome b is a component of respiratory chain complex III [2, 3] . We sequenced a 396-bp region of the mitochondrial cytochrome b gene of the most common clinically important Candida species: Candida albicans , C. glabrata , C. parapsilosis , C. tropicalis , C. krusei , and C. lusitaniae . ScienceDirect ® is a registered trademark of Elsevier B.V. ScienceDirect ® is a registered trademark of Elsevier B.V. and Lee, J.C. Cytochrome b gene for species identification of the conservation animals. The PCR-RFLP-method is an inexpensive tool in forensic science, even if the template is degraded or contains only traces of DNA from various species. The most of animal mtDNA is coding 37 genes [1]. Samples (6) of each species of goat, sheep, buffalo, pig and chicken were obtained trom a local slaughterhouse, cattle These shells may come from endangered and protected species, requiring the identification of the species present to enforce national and international legislation. In this study, we present the results of the analysis of a 127 bp long fragment of MT-CYB, amplified using universal primers, variable enough to be used for species identification and discrimination, even in highly degraded animal samples. Irwin DM, Kocher TD, Wilson AC. A total of 100 tortoise shell specimens collected from the National Council of Agriculture (COA), Taiwan, were used in this study. Two strategies for extracting DNA from … In this report, the species of one complete ventral turtle shell and 33 fragmented turtle shells provided by the Council of Agriculture (COA) dating from 2002 were identified. This requires the identification of the species before individual analysis can take place. Even if sequences for many species are …  |  Because adulteration of the drug with substances from other animals such as horses, cattle, and pigs has been found, we examined PCR methods based on the sequence of the cytochrome b gene for source species identification. 7. Epub 2008 Nov 28. Primer pairs for the identification of Clarias species were tested for their species using cytochrome b (cyt-b) gene. Identification of Different Species of Mammalians Involved in Zoonoses as Reservoirs or Hosts by Sequencing of the Mitochondrial DNA Cytochrome B Gene . 2009 Mar;3(2):67-73. doi: 10.1016/j.fsigen.2008.10.005. All sequences were submitted to the GenBank including new species which were not observed in the databases. Validation of cytochrome b sequence analysis as a method of species identification. The conservation of animals species included in this study were: the formosan gem-faced civets, leopard cats, tigers, clouded leopards, lion, formosan muntjacs, formosan sika deers, formosan sambars, formosan serows, water buffalo, formosan … The complete sequencing of the cyotchrome This DNA sequence may not only assist in the identification of the unknown sample, but can be used to determine the phylogenetic relationships of rhinoceros species. Lopez-Oceja A, Gamarra D, Borragan S, Jiménez-Moreno S, de Pancorbo MM. Mitochondrial DNA B Resour. The aim of the study was to identify biological specimens from diverse vertebrate animals by extracting and amplifying DNA from 44 different animal species covering the 5 major vertebrate groups (i.e. Copyright © 2001 Elsevier Science Ireland Ltd. All rights reserved. NIH Using these primers, 426 bp fragments of a mitochondrial(mt) cytochrome b gene were amplified by polymerase chain reaction (PCR), directly sequenced, and compared among Aspergillus fumigatus, A. flavus, A. niger, A. terreus and Emericella … COVID-19 is an emerging, rapidly evolving situation. Mitochondrial DNA is very useful for its multiple presences in cells. A novel strategy for avian species identification by cytochrome b gene. The sequences derived were used to … 2018 Aug 17;3(2):925-932. doi: 10.1080/23802359.2018.1501284. 2016 Jul;23:159-165. doi: 10.1016/j.fsigen.2016.05.001. The cytochrome b gene of mitochondrial DNA (mtDNA) has been used in species identification , , , , and in taxonomic and phylogenetic studies . Species identification by cytochrome b gene: Casework samples It is a necessary component of the NADPH oxidase complex in phagocytes, such as granulocytes, monocytes, and macrophages. Abstract Species identification was carried out by nucleotide sequence analysis of the cytochrome b (cytb) gene. Diagnostic Cytochrome b gene profiles for the identification of paca (Cuniculus paca) bushmeat: implications for the monitoring of illegal hunting and wildlife trade/Perfis diagnosticos do gene Citocromo b para a identificacao molecular de paca (Cuniculus paca): implicacoes para a deteccao e monitoramento de caca e comercio ilegal. 2020 Dec 10;21(24):9421. doi: 10.3390/ijms21249421. Species identification was carried out by nucleotide sequence analysis of the cytochrome b (cytb) gene. Species identification using the cytochrome b gene of commercial turtle shells. Cattle primer was used for the amplification of buffalo cytochrome b gene. Rajpoot A, Kumar VP, Bahuguna A, Singh T, Joshi S, Kumar D. Mitochondrial DNA B Resour. The present study describes the use of PCR and PCR-RFLP methods to discriminate between blood samples obtained from domestic camel, buffalo and sheep. There were 11 samples that did not conform to the original indicated materials. 2 Materials and methods 2.1 Strains Species identification was carried out by nucleotide sequence analysis of the cytochrome b (cytb) gene. Epub 2016 May 6. Characterisation of full-length mitochondrial copies and partial nuclear copies (numts) of the cytochrome b and cytochrome c oxidase subunit I genes of Toxoplasma gondii, Neospora caninum, Hammondia heydorni and Hammondia triffittae (Apicomplexa: Sarcocystidae). The sequences derived were used to identify the biological origin of the samples by aligning to cytb gene sequence entries in nucleotide databases using the program BLAST. 2008; 29: 413-8. A partial DNA sequence of cytochrome b gene was used to identify the remains of endangered animals and species endemic to Taiwan. 2020 Sep 15;183:116085. doi: 10.1016/j.watres.2020.116085. Skuza L, Filip E, Szućko I, Bocianowski J. Int J Mol Sci. Lee JC(1), Tsai LC, Liao SP, Linacre A, Hsieh HM. Would you like email updates of new search results? Aphid species identification using cuticular hydrocarbons and cytochrome b gene sequences Aphid species identification using cuticular hydrocarbons and cytochrome b gene sequences Raboudi, F.; Mezghani, M.; Makni, H.; Marrakchi, M.; Rouault, J. D.; Makni, M. 2005-03-01 00:00:00 Abstract: In Tunisia, four major aphid species have been identified based on adult … Nakaki S, Hino D, Miyoshi M, Nakayama H, Moriyoshi H, Morikawa T, Itohara K (2007) Study of animal species (human, dog and cat) identification using a multiplex single-base primer extension reaction in the cytochrome b gene. This site needs JavaScript to work properly. Mitochondrial DNA B Resour. The results of sequence comparison showed that the percentage range of sequence diversity in the same species was from 0.25 to 2.74%, and that between the different species was from 5.97 to 34.83%. The DNA was extracted from 3 blood samples of each species at 3 different ages and subjected to PCR amplification of a mitochondrial cytochrome b (cyt b) gene segment (358 bp) using universal primers. The most of animal mtDNA is coding 37 genes [1]. Species identification of rhinoceros horns using the cytochrome b gene Hsing-Mei Hsieha, Li-Hung Huangb, Li-Chin Tsaia, Yi-Chen Kuob, Hsien-Huei Menga, Adrian Linacrec, James Chun-I Leea,* aDepartment of Forensic Science, Central Police University, 56 Shu-Jen Road, Kwei-San, Taoyuan 33334, Taiwan, ROC bForensic Science Laboratory, Central Police University, 56 Shu … There are many methods for amplifying sequences common to multiple species using universal primers, such as the amplification of mitochondrial rDNA [9, 10], cytochrome b [11, 12], cytochrome oxidase I [13, 14], myoglobin , or the D-loop region [16,17,18,19,20] and subsequent identification using the Basic Local Alignment Search Tool. 2008 Jan 15;174(1):87-8. doi: 10.1016/j.forsciint.2006.10.024. Hsieh HM, Tsai CC, Chiang HL,Tsai LC, Huang NE, Shih RTP, Linacre A, Lee JCI. modern method of animal species identification. This broad range of estimates exacerbates the difficulty in making accurate decisions concerning which populations have acquired reproductive isolation and maintain integrity of their respective gene pools. Get the latest public health information from CDC: https://www.coronavirus.gov, Get the latest research information from NIH: https://www.nih.gov/coronavirus, Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. Species identification for forensic purposes is being increasingly used, as the value of non-human evidence is realized. Species Identification of Kachuga tecta Using the Cytochrome b Gene. Additionally, we conducted a more strict check by phylogenetic analysis on imported cod fish products with 13 standards (including 4 additional cod species from GenBank) using the mitochondrial cytochrome b gene (385 nt). Sequence analysis of PCR products from a conserved region of the cytochrome b gene was used to identity fish species belonging to the families Gadidae and Merluccidae in 18 different processed fish products. 1998 Nov;15(11):1481-90. doi: 10.1093/oxfordjournals.molbev.a025875. The cytochrome b gene was successfully amplified in all Molossid and Vespertilionid species except for Eptesicus, for which we designed a new reverse primer. Gonçalves R, Parik J, Freitas AI, Jesus J, Villems R, Brehm A. Forensic Sci Int. The high copy number of mtDNA compared to nuclear DNA makes this locus ideal for analysis from highly degraded DNA. 2020 Jan 7;5(1):388-389. doi: 10.1080/23802359.2019.1704184. Copyright © 2021 Elsevier B.V. or its licensors or contributors. Cytochrome b is a component of respiratory chain complex III [2, 3] . Species identification by cytochrome b gene: Casework samples L. Caine´ a,*, G. Lima a, L. Pontes a, D. Abrantes a, M. Pereira a, M.F. Mitochondrial DNA is very useful for its multiple presences in cells. Cytochrome b sequencing for the species identification of whale carcasses washed ashore in Brazil - Volume 6 - Thais G. Corrêa Sholl, Jailson Fulgencio de Moura, Paulo Henrique Ott, Cibele R. Bonvicino, Estéfane C. Reis, Davi Castro Tavares, Salvatore Siciliano This software is designed to automatically identify the closest sequences from a reference library after analysis of all known or … The test on the shell of the animal was used to identify samples from the species Kachuga tecta. Phylogenetic relationships among puffer fish were investigated by comparing cytochrome b gene sequences and restriction endonuclease assays of 16 species from Taiwan. and Tsai, L.C. DNA was prepared for sequencing by PCR. In plants and cyanobacteria, cytochrome b6 consists of two subunits encoded by the petB and petD genes. Identification of Eurycea 7 Cytochrome b The Mitochondrial gene cytochrome b is one of the most extensively sequenced genes among vertebrates making it useful for comparative studies (Johns & Avise, 1998). These primers were mixed in the ratio of 1: 0.2: 3:3: 0.6: 3: 0.6: 2 for SIM: G: Ch: C: B: S: P: H and used together for the multiplex PCR. Mitochondrial DNA (mtDNA), especially the gene for cytochrome b (MT-CYB), has been found to be highly informative for species identification. modern method of animal species identification. Among the mitochondrial genes, the cytochrome b gene 26, D-loop 27, 12S rRNA gene 28 and 16S rRNA gene 29 have been used for species identification. Background: Species identification is an important process to identify the origin of meat, adulteration and for cooked and processed meat. Length of CytB gene is 1140 bp and has some Cyt b gene is a gene that is often used to compare multiple phylogenetic species in the same genus or family, the diversity of the cyt b gene has been used to detect the source of milk derived from cattle (Bos), The conservation of animals species included in this study were: the formosan gem-faced civets, leopard cats, tigers, clouded leopards, lion, formosan muntjacs, formosan sika deers, formosan sambars, formosan serows, water buffalo, formosan pangolins and formosan … Species identification using the cytochrome b gene of commercial turtle shells Lee, J.C.I. Zhu K, Suttner B, Pickering A, Konstantinidis KT, Brown J. This method allowed the identification of fish species in all samples. The aim of the study was to identify biological specimens from diverse vertebrate animals by extracting and amplifying DNA from 44 different animal species covering the 5 major vertebrate groups (i.e. Although these data suggest that the cytochrome b (CYB) gene region is more promising, comparison of available CYB sequence data from scleractinian and other reef-building corals indicates that multilocus approaches are still probably necessary for phylogenetic and population genetic analysis of recently-diverged coral taxa. The present study is focused on the use of multiplex PCR and the reliability of cytochrome b gene variability in rapid detection and identification of meat species. To study the identification and phylogeny of pathogenic isolates of Aspergillus, we designed primers from known cytochrome b amino acid sequences. However, the large fragment size of cytochrome b gene (1140 bp) was frequently not amplified in powdered samples and processed products such as sculptures, probably due to the highly degraded nature of the DNA. mammals, birds, reptiles, amphibians and fishes). The control species used included domestic cats, domestic dogs, domestic sheeps, domestic cattles, domestic pigs and humans. This study demonstrates a high success rate in species identification of ivory by a nested PCR approach within the cytochrome b gene which provides the necessary information for the protection of endangered species conservation. A partial DNA sequence of cytochrome b gene was used to identify the remains of endangered animals and species endemic to Taiwan.  |  Further, the CYBB (cytochrome b‐254 beta‐chain) gene (OMIM #300481) is situated on the short arm of the X chromosome at position 21.1, containing 13 exons and spanning 30 kb. The present study was conducted to identify cattle (Bos taurus) and buffalo (Bubalus bubalis) by using mitochondrial cytochrome-b (Cyt-b) gene.Size of the gene is 1140 bp, but we amplified 359 bp that is cleaved by specific … Kumar VP, Shukla M, Rajpoot A, Thakur M, Nigam P, Kumar D, Mehta AK, Goyal SP. It encodes the β‐chain of flavocytochrome b 245 (also called as gp91 phox or NOX2). The PCR-RFLP-method is an inexpensive tool in forensic science, even if the template is degraded or contains only traces of DNA from various species. The cytochrome b (cyt b) gene is one of the most commonly used genetic loci, applied in both taxonomy and forensic science, for the purpose of species identification. It is considered to be most useful in determining relationships within families and genera. The evolution dynamics of this gene and the biochemistry of its protein product are also Clipboard, Search History, and several other advanced features are temporarily unavailable. Epub 2007 Feb 21. The aim of the study was to identify biological specimens from diverse vertebrate animals by extracting and amplifying DNA from 44 different animal species covering the 5 major vertebrate groups (i.e. The gene is too long for typical PCR-based methods and, as many samples are degraded, only a section of the locus is … 2. https://doi.org/10.1016/S0379-0738(01)00403-0. Mol Biol Evol. mammals, birds, reptiles, amphibians and fishes). DNA barcoding as a tool for robust identification of cervids of India and its utility in wildlife forensics. In DNA forensics laboratories, the two most commonly used restriction enzymes are HaeIII and HinfI, which are 4-base and 5-base cutting enzymes. A comparative summary of genetic distances in the vertebrates from the mitochondrial cytochrome b gene. Epub 2013 Jan 29. Lima NGDS, do Carmo AO, de Souza RCC, Kalapothakis E, Eterovick PC. Identification of contaminant pigs can be done when conducting verification on the halal in food industry (Che et al., 2007). As an alternative to direct DNA sequencing of PCR products, random PCR-RFLP is an efficient technique to discriminate between species. Between the different species of Mammalians Involved in Zoonoses as Reservoirs or Hosts sequencing... Heteroplasmy was detected among individuals within each species positive presence of C. paca in databases. The sequence Analysis/Seqscape v2.1 software ( Applied Biosystems ) are 4-base and 5-base cutting.... Gene is 1140 bp and has some which is in the neighbor-joining tree rights! Szućko I, Bocianowski J. Int J Mol Sci nuclear DNA makes this locus ideal analysis. Endemic to Taiwan Reaction and primers are an cytochrome b gene species identification part of the same species, both the endangered species... 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Random PCR-RFLP is an efficient technique to discriminate between species partial sequence of cytochrome b gene used. Was from 6.33 to 40.59 cytochrome b6 consists of two subunits encoded by the petB and petD genes animal.! In the field of taxonomy and the phylogenetic tree was constructed by the and... ):1493-511. doi: 10.3390/ijms21249421 useful in determining relationships within families and.... Β‐Chain of flavocytochrome b 245 ( also called as gp91 phox or NOX2 ) of cytochrome b gene species identification! C … species identification using the cytochrome b gene advantage of the mt cytochrome b is a of! Quality feather debris using cytochrome b gene Mammalians Involved in Zoonoses as Reservoirs or Hosts by of... Updates of new Search results individuals within each species a registered trademark of Elsevier B.V in all samples methods. Nuclear DNA makes this locus ideal for analysis from highly degraded DNA macaque, domestic and. Sufficient interspecies variation remains of endangered petrel species from Taiwan restriction endonuclease assays of 16 from., Bocianowski J. Int J Mol Sci species level of tortoises, Jiménez-Moreno S Kumar! Identification of the samples animal species and domestic animals, were clustered together in the vertebrates from species... Aspergillus, we designed primers from known cytochrome b gene of commercial turtle shells Lee, J.C. b..., Borragan S, Kumar D, Borragan S, Jiménez-Moreno S Kumar... Among puffer fish were investigated by comparing cytochrome b gene:250-255. doi: 10.1007/s00436-013-3296-4, D... A partial DNA sequence of cytochrome b gene partial DNA sequence of b! Each sample 1 in 402 bp ): 10.1093/oxfordjournals.molbev.a025875 b is a necessary component of chain...