This breed of rat was first produced by the Sprague Dawley farms in Madison, Wisconsin (later to become the Sprague Dawley Animal Company). The strain was created by R. W. Dawley in 1925, from a hooded male hybrid of unknown origin and an albino female (probably Wistar), and was crossed with the female’s progeny for 7 generations.The SPRAGUE DAWLEY® rat is an albino outbred rat with an elongated head … Other aspects of health were evaluated, such as body weight (20% body weight loss at any time point warrants euthanasia), body condition score, tumor ulceration, and ensuring that the tumor does not interfere with locomotion or normal activities. To detect T, B, and NK cells in SRG rats, flow cytometric analysis was performed on splenocyte, thymocytes, and whole peripheral blood using a BD LSRII. https://doi.org/10.1371/journal.pone.0240169.g002. In the current exploration, different parts of bitter gourd were evaluated for their cholesterol lowering potential in cholesterol fed Sprague dawley rats. Compared to mice, rats are bigger, generally more aggressive, and more resistant to various ailments. for a 200 g animal, the tumor cannot weigh more than 20 g = 20,000 mm3). VCaP and HCT-116 were gifted to Hera BioLabs in April 2017. Flow cytometry dot plots show representative data from one WT and one SRG rat each. Sprague Dawley Rat High Fat T Increases Pain Behaviors In Rats With Or Without Nutrients full text calorie restriction modulates 1209 s7 article 03 sprague dawley rats a model of successful heart aging sciencedirect an weights of sprague dawley rats and age table safety essment and single dose toxiiics of the flavouring Other criteria warranting euthanasia are ulceration on greater than 25% of the surface or perforation in any size ulceration that causes exudate or infection. These data demonstrate that SRG rats support the growth of human cancer cell lines with favorable take rates and growth kinetics for preclinical efficacy studies. Writing – original draft, Affiliation There were no changes observed in motility or food intake in tumor bearing animals. Analyses of Prostate Specific Antigen (PSA) in the serum showed a significant positive correlation to tumor volume in SRG rats with a 0.92 coefficient of correlation between serum PSA levels and tumor volume (Fig 4D). Humane endpoints take the following into consideration: tumor diameter or tumor weight vs. body weight, tumor ulceration, animal weight, body condition score, animal mobility and activity. Nurul Iftitah Musameh, Siti Rosmani Md Zin, Normadiah M. Kassim Department of Anatomy, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia Abstract Genistein (Gen), is commonly consumed phytoestrogen among Asian and known to exert weak estrogenic effects. Initial engraftment of the patient tumors resulted in tumor volumes of 4,000 mm3 by 75 days post-inoculation and tumor growth rate in SRG rats increased through serial passages (Fig 5A and S3A–S3F Fig). By 10 days post-inoculation, tumor take rate was 100% in both NSG mice and SRG rats. All cells lines underwent monthly testing for mycoplasma contamination (Lonza, LT07-710) and STR testing at later passages. Knowing this cell line engrafts and grows poorly in the mouse and taking advantage of the size of the rat, we inoculated SRG rats with 10 x 106 cells (~40,000 cells/cm2 BSA). (Unpaired t-test, p-values: ** <0.01). The last NSG mouse that achieved a tumor size sufficient for drug testing occurred at 16 days post-inoculation but had significantly slower growth such that it was under 900 mm3 at 40 days post-inoculation. DAB substrate was applied followed by counterstaining with hematoxylin. Published success rates for NSCLC-PDX establishment subcutaneously in mouse models are in the range of 20–40% which means even at best, the models fail for more than half of the patients [8–10]. Our studies have demonstrated that SRG rats support growth of a wide array of human cancer cell lines, including ones that have poor uptake or variable growth kinetics in available mouse models. Rats were . Sprague-Dawley and Wistar are the two most frequently used rat models. For transplantation, 5-10x106 VCaP cells, 2x106 HCT-116 cells, 5 x106 MIA-PaCa-2, 10 x106 786-O cells, or 5x106 HCC1954 cells were injected in each animal. A) Tumor growth curve shows multiple passages of the patient derived lung tumor from one patient and subsequent passages in the SRG rat. Food and water were provided ad libitum and nestlets or virgin kraft paper were provided in all cages for enrichment. The SRG rat spleen was devoid of mature B cells, as assessed by cell surface markers CD45RA (B220) and IgM (Fig 1D–1F). R. W. DAWLEY created the strain SPRAGUE DAWLEY® in 1925, and it was bred at SPRAGUE DAWLEY farm. All the cells were grown in a humidified incubator at 37°C with 5% CO2. We are currently using the SRG rats to establish PDX models from different cancer types in order to study the engraftment rate and time frame from patient tumor resection to preclinical study. "Rats, Sprague-Dawley" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings).Descriptors are arranged in a hierarchical structure, which enables searching at various levels of specificity. H) Quantification of data, n = 3, error ± SD. Tumors were monitored once daily for signs of ulceration. No, PLOS is a nonprofit 501(c)(3) corporation, #C2354500, based in San Francisco, California, US, https://doi.org/10.1371/journal.pone.0240169. Surgically resected NSCLC tissue from nine patients were obtained from the Biospecimen Procurement and Translational Pathology Shared Resource Facility of the University of Kentucky Markey Cancer Center (P30CA177558) in collaboration with Dr. Mark Evers under an approved University of Kentucky IRB application. Blood was collected from SRG rats in Clot activator SST microtainers prior to inoculation of VCaP cells and then weekly throughout the study. Indicates specific parameters that are monitored ( Table 1 ) mutation appeared in a high-quality journal and their molecular histological. ( ThermoFisher scientific ) shafts shaped like a lance head ( “ lanceolate )! Period prior to onset of shock of human cancer cell lines and NSCLC-PDX samples in the serum of rat... If it is handled improperly ) tumor growth curve shows multiple passages for each line in addition to existing!, Wednesday, and TTF1 25 ] Change trends of organ weight background data in Sprague Dawley reproductive. 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