If it was that easy, every mushroom grower would just be using one ultimate substrate! If youre lucky, you may get a few extra flushes of mushrooms from your compost pile or garden. Yield: 6-12 lbs from a 25 lb straw log, up to 3 lbs from a 5 lb supplemented sawdust fruiting block. Choose the substrate for mushroom cultivation. If words like inoculation or mycelium seem overwhelming right now, our article How Do Mushrooms Grow? of fresh mushrooms from 1 lb. Which means you can usually pick it up for free (or extremely cheap). From here if you continue to give the mycelium the stable fruiting condition they will develop into mushrooms so just be patient and prepare to be amazed! But since its made of partially-digested food, it makes an easy food source for all kinds of different lifeforms. Generally speaking, they prefer these types of substrate because they are cost-effective in large quantities. Coco coir, logs and cardboard similarly dont need to be sterilized. 85-90 per cent. The mushroom yield is simply the amount of fresh mushroom fruit you can obtain from a growing space and substrate. There are a few ways that you can sterilize a substrate without using a pressure cooker. Spray a 10% bleach solution in the air. Thanks for the kind words! Why Is Pasteurization or Sterilization Necessary? Wait until the bulk substrate is engulfed in the white Mycelium and your substrate is completely covered white. And what of all this do Ireallyneed to know? But as you get more proficient at growing mushrooms- and especially if you want toturn your hobby into a business you should be increasingly concerned about how many mushrooms you can grow with the substrate you have. Copyright 2023 Garden Bench Top. The mycelium uses the growing medium as a source for food and energy to quickly grow and begin fruiting. Agaricus and Specialty Mushroom Sales, Price, and Value . After few days when the This will yield anywhere from 1-5 pounds of mushrooms, depending on the conditions in which they are grown. GOOD, because mycology and growing mushrooms from mycelium is an addictive hobby. . The PF Tek is probably the best substrate for beginners, even when growing gourmet varieties. Diversified Farming and Food Systems. What you will find is most mushroom growers will include a few different types of growing mediums in their cultivation of mushrooms. How To Make A Casing Layer For Growing Mushrooms. This is necessary for the mycelium to colonize well. Now weight the mycelium (mushroom spawn) and use the desired quantity (usually 2-3% = 2-3 kg / 4.4.-6.6 lbs to 100kg /220 lbs of compost) 4. It can be purchased at farm stores or most places where you can find animal feed or bedding. Once that is ready, the substrate needs to be sterilized or pasteurized. If you contact local coffee shops, they will often save their spent coffee grounds and give them to you for free. Ive read in many places that certain species won't fruit a second time however for me they have so its always worth trying if you dont need the growing vessel immediately. A 500-square foot growing space should produce 12,000 pounds of mushrooms annually. No one knows what will happen with your trays, we cannot predict what your genetics will do. HiTony The website is very informative.. This part of the process helps give your mycelium a head-start in getting established. King_Weezer 7 yr. ago. West Lafayette, IN 47907. If its wet weight than that yield isnt bad at all! Soaking logs before planting is an effective way to increase yields. Some substrates may also start to burn. Let's go and talk about the last point - the spawn rate. Different mushrooms, and different strains of the same species, will have a wide range of potential biological efficiencies. Both pasteurizing and sterilizing substrate involve heating up the substrate in an attempt to destroy existing bacteria or fungi, but pasteurization heats substrate up to 185 degrees F, while sterilizing exposes the substrate to temperatures higher than 250 degrees F. The purpose of pasteurization is to get the substrate fairly contaminate-free and gives any mushroom culture that you introduce a good head-start. If youre fermenting a whole straw bale youll probably need to submerge it for longer, about two weeks. Advertisement cookies are used to provide visitors with relevant ads and marketing campaigns. Holes are then sealed with wax to prevent contamination. The combination of poor yield and difficulty may explain why P. cyanescens is grown less frequently than some other psilocybin containing mushrooms. Reddit or eBay can also be a good source but make sure the seller has good reviews and you have exhausted other avenues first. Wheat Straw (or hay) is a very popular choice for a mushroom growing medium. In this article, I want to give you a basic introduction into growing mushrooms from mycelium, this won't be an extensive or detailed post but several links within this post will branch off should you require any in-depth guidance. BLUE OYSTER B.E. It is natural to wonder how many mushrooms (or the yield of mushroom) you are going to harvest from your mushroom growing kits. Human being too Many blessings Kpg. Or you may need to drive the used substrate to a city depot that handles yard waste, compost and other recyclables to drop it off yourself. This cookie is set by GDPR Cookie Consent plugin. These include your fruiting conditions, the type of mushroom you're growing, humidity, ventilation, etc. This provides a blank canvas for your mushroom mycelium to thrive. Answer (1 of 6): Hello, Oyster mushrooms are possibly the easiest variety of mushrooms to grow. This website uses cookies to improve your experience while you navigate through the website. Magic Mushrooms (aka psilocybe cubensis), and. Selecting the right substrate and preparing it correctly for the specific type of mushroom that youre growing is critical for your success. It is one of those substrates that seems to tick all the right boxes when it comes to creating the perfect cultivating environments for mushrooms. We can offer wholesale rates if you are interested in a minimum purchase of 20 blocks of the same mushroom species. As a general rule we find for every 30 pounds (around 13 kilograms) of total mushroom substrate materials, you can expect to grow around 7.5 pounds (around 3 kilograms) yield of . One great benefit is that the brewing process pasteurizes the grounds and you can start growing in them pretty much right away. Bottless 7 yr. ago. That being said, it is sometimes better to go for a larger yield on the first flush, and future flushes may have diminishing returns. Table 3. Cardboard retains moisture well while the corrugations allow for good air exchange. Lions Mane: 1Lb per 5lbs substrate block: Reishi: 1lb per 5lbs substrate block: Shiitake: 2lb per 5lbs substrate block: Yellow Oyster: 3lb per 5lb . Theres no easier way to mix substrate than simply mixing it up with your hands. In a way, increasing your spawn:bulk substrate ratio is a way to increase your nitrogen content, and is a form of supplementation. Because the calculation uses the weight of dry substrate, it is possible- and actually quite common to achieve a biological efficiencygreater than 100%. Luckily, top-performing strains have been carefully developed and optimized over the years to produce good and reliable yields. We prefer to use a heating method thats both quick and organic. It might not seem like a very appealing habitat to humans. This is a tried and tested combination. Simply put, a yield of 1 lb. With this method creating an honey liquid culture is pretty simple if you are using the correct technique, but I won't be going into detail about that in this post. Hey Matthew! The method we use (albeit rudimentary) is to essentially soak the substrate in a hot water bath. Supplementation is usually achieved by adding bran to the substrate, typically wheat bran or oat bran. When spawn, substrate, and optimal conditions are used, one can easily harvest 600-900 grams . Adding too much supplementation, however, will actually have detrimental effects. When youre fermenting, youll need to predict what your substrate needs will be a week or more out. Check out our YouTube for new content weekly! A good example of this is straw. Here at Grocycle, we started off by growing on coffee grounds and weve been doing it for years ever since. In doing so, the mushrooms will fruit for longer periods of time and more consistently, rather than big flushes of mushrooms. When you squeeze a handful of the mushroom growing substrate, only a few drops of water should come out. Some farmers also mix hardwood pellets with different ratios of soybean hulls for a similar effect. A mushroom log can be used to grow mushrooms at home. If youre in the United States, you can buy bags of this already made from Mushroom Media Online. So 2 and a half cups of wheat or oat bran can be added to provide extra nutrients for the mycelium. Before a substrate can be inoculated with mushroom spores or mycelium, first it needs to be prepared. These are small pieces of wooden dowel that are colonized with mycelium that get hammered into the holes. As weve alluded to throughout this guide, different kinds of substrates are best suited to different mushroom species. Think about the process of fermenting foods like sauerkraut, kimchi or kombucha. Functional cookies help to perform certain functionalities like sharing the content of the website on social media platforms, collect feedbacks, and other third-party features. Topic of the day at the Garden Bench Top is mushroom substrate. Some varieties of fungi like truffles even prefer to grow on the roots of living trees, which makes them notoriously difficult to grow in a commercial setting. Different species of mushrooms have their own preferences. Different strain of king oyster (Pleurotus eryngii) respond differently depending on the substrate used for cultivation, supplements and environmental factors. Liquid mushroom culture makes inoculating substrates easier because the mixture is inoculated directly onto a substrate of your choice. When you first start out growing mushrooms, you might not be all that concerned about how many mushrooms you get. Yield, mushroom size and time to production of Pleurotus cornucopiae (oyster mushroom) grown on switch grass substrate spawned and supplemented at various rates. Place the closed bag in a dark, warm area around 20 - 24C ( 68 - 75F) and leave it to incubate. King Oyster Mushrooms harvested at different stages of growth. Its where the mushrooms will get all of their nutrients while growing. Step 1: Make or buy mushroom grain spawn. So if we consider 500-kilo grams as average yield per crop for 9 crops it will be 9 x 500 4 500. Yield was calculated as weight (kg) of mushrooms produced per kilogram of substrate. Some species like oyster mushrooms can even take hold and spread and youll get free mushrooms growing in your garden year after year. Another benefit wheat straw has going for it is that it can be purchased in bulk, and is affordable for the mushroom growing hobbyist. Logs are often used to grow shiitake and other varieties of mushrooms outdoors. u/Rodney_Ogg posted this in r/shroomers recently. Most substrates (like sawdust or straw) need to have additional materials added to them to reach this threshold. I was wondering if you might be able to provide the BEs for Cordyceps, Turkey Tail and Chaga? Next, you need to add your lion's mane spawn at a 5% spawn rate to wet substrate per bag, mix them together and close the top of the bag with a rubber band or a tie of some kind. For example, adding manure to hardwood sawdust. Some types of oyster mushrooms will also grow well on wood. Unfortunately, I dont really have much experience with growing button mushrooms, although it is something I am definitely going to try. You can use horse, chicken, cow, or other types of manure for mushrooms. This can vary depending on the type of mushroom, the growing conditions, and the age of the mushrooms when harvested. Yes, there should be a difference in yield, although I do not have hard data on the subject. Most of the mushrooms that grow on wood chips (above) will also grow with wood pellets. greater nutritional value for the mushroom spores to consume, providing a better foundation for a success flush of mushrooms. easier to source and bulk out your mushroom growing kits. This cookie is set by GDPR Cookie Consent plugin. While the economic yield (EY) is defined as the yield per block after removing the . A mushroom culture starts when the hyphae of two compatible germinating spores mate and start to grow out as mycelium. Barley straw. Nowadays many cities have a free composting program. Here is the process we follow for our making our master's mix for our production blocks. Experimenting with different substrate mixes is something that you can try once youve been growing mushrooms for a while. The fact that the calculation uses the dry weight of the substrate throws many growers off, since much of the weight of a fruiting block or straw log will be water. I have 2 pounds of sterilized rye seed substrate, if I use the casing method and everything goes fine how many mushrooms in dry weight can I expect from that amount of substrate? Generally speaking, gardeners use perlite as one (of many) components in their garden soil mixes. But what about people who are growing mushrooms in the city? The averages are based on facilities that represent more than 30% of US mushroom production. You can also add in a bit of sawdust to the mix for a bit of variety. Sterilization involves exposing the substrate to temperatures higher than 250 degrees F and also involves placing the substrate under pressure. Our bins hold up to 20 pounds of substrate and 10 pounds of grain (approx 20 quarts). For a standard brick of dried coco coir (about 1.5 lbs) add eight cups of dry vermiculite, along with 16 cups of boiling water. The cost per pound of mushrooms can vary by type. This guide will take you through the general process of the mushroom growth cycle, and how to construct your kit in a step-by-step process. There are a wide variety of edible and medicinal wood-loving mushrooms. For instance, having a higher ratio of 1:8 (1 part spawn:8 . Using substrate to grow mushrooms is the equivalent of using soil to grow plants. Vermiculite is a yellowish-brown mineral thats used to retain moisture and is also widely available at garden centers everywhere. If youve been growing mushrooms for any length of time, youll soon end up with big piles of spent substrate. If youre pasteurizing, your substrate can be ready to use in just a few hours. Besides selecting a substrate to use, deciding when its appropriate to either pasteurize or sterilize the material is another important part in learning how to make mushroom substrate. Now you know how to create an ideal substrate for your mushrooms. To do this method, soak your substrate in a bath of hydrated lime-treated cold water for 24 hours. 50 (1) Blue Oyster Liquid Culture Syringe (10cc). :100-200% Yield:1.5-2.5 lbs on a supplemented sawdust fruiting block. if(window.strchfSettings === undefined) window.strchfSettings = {};window.strchfSettings.stats = {url: "https://mistermemo.storychief.io/en/mushroom-substrate?id=1786563703&type=2",title: "Mushroom Substrate",id: "845dd9c8-36a3-470c-8676-3915d655e1cd"};(function(d, s, id) {var js, sjs = d.getElementsByTagName(s)[0];if (d.getElementById(id)) {window.strchf.update(); return;}js = d.createElement(s); js.id = id;js.src = "https://d37oebn0w9ir6a.cloudfront.net/scripts/v0/strchf.js";js.async = true;sjs.parentNode.insertBefore(js, sjs);}(document, 'script', 'storychief-jssdk')). Its available for sale in most garden stores. PINK OYSTER B.E. Effect of different substrates on the yield of C. indica . The main reason is to prevent bacteria or other plant organisms that your substrate may be housing from growing and eventually competing against your mushroom spawn. Hi, thank you for this very informative blog! However, I have done a little bit of research and created this table. Not at the moment, just THRIVE 6 (Chaga, Reishi, Turkey Tail, Maitake, Lions Mane and Cordyceps) Hero (pure Cordyceps Militaris), Electric (Pure Lions Mane) and RECHARGE (pure Chaga) Stay tuned though! Generally, you can expect to get about 1/2 to 1 pound of mushrooms per pound of substrate. Necessary cookies are absolutely essential for the website to function properly. The cookie is used to store the user consent for the cookies in the category "Performance". The cookie is set by the GDPR Cookie Consent plugin and is used to store whether or not user has consented to the use of cookies. 1:1 and a higher ratio utilizes more substrate than spawn. That way you wont need to buy mushroom spawn from suppliers and can have a truly self-sufficient operation. Become a VIP for discounts, giveaways and more. Agaricus Mushroom Area in Production and Yield - States and United States: 2018-2019, 2019-2020, . YELLOW OYSTER B.E. Mycelium is a living organism, therefore if damaged the mycelium won't be able to spread throughout the substrate and this will result in low mushroom yield. amzn_assoc_marketplace = "amazon"; A process called tyndallization or fractional sterilization can also be used. Should it be more than that? If your city doesnt offer any way to recycle your substrate, you can reach out to nearby farmers. Here are some of the most common substrates that mushroom growers are currently using. Let's look at some different mushrooms and their BE's, and expected yields. There are a wide range of materials that you can use as a substrate to grow mushrooms. Then mix everything up, put the lid back on and allow it to cool for four hours before inoculating. There are several ways to pasteurize mushroom substrate. 3. The spawn ratio that we recommend is two pounds of colonized grain spawn per five pounds of pre-pasteurized mushroom compost. REISHI B.E. Bulk orders require notice and prep. If youre working with large quantities of straw, it can be easier to use a weed-whacker in a garbage bin to quickly shred the material. Pasteurizing your mushroom growing substrate is the equivalent of pulling as many weeds as you can out of the garden before planting vegetables. The information provided by this website or this company is not a substitute for a face-to-face consultation with your physician, and should not be construed as individual medical advice. For full sterilization, a pressure cooker or similar piece of equipment is needed. The total mushroom yield is calculated by counting the total weight (in grams) of harvested mushroom fruit from all flushes. The thing about wood chips is you want to be particular about the type of wood you select. 1. If you have a particular species of mushroom that you are looking to grow in your backyard, you can tailor your substrate to enhance you chances of growing a successful crop of mushrooms. Straw is another cheap and effective material to use as a substrate. However for some types of mushrooms, like common button mushrooms, its necessary. Transactions ASAE. While it is languishing in a syringe (minimum for 24 hours) and waiting for inoculation, it's time to take care of the substrate for future magic . Please, reply if you could! You can either grow in a substrate composed of 100% straw or add supplements to provide additional nutrients. Q: How long does it take to grow? Assistant Program Leader. This is ideal for DIY mushroom farmers, because you can leisurely enjoy fresh mushrooms for weeks, rather than just days. This might leave you asking yourself What can I do with an old mushroom substrate? The most common choice for mushroom growers is to compost it. Some people have very complicated mushroom growing setups that involve cleanrooms with negative air pressure and expensive equipment. Water then needs to be added to reach field capacity. I use straw rather than hay. Wood Chips is great of growing the following varieties of mushrooms: We thought it would be sensible to talk about wood pellets as another option for mushroom substrate. The purpose here is to give an overview of the process. Average kWh's per pound of mushrooms produced for Phase III through harvest Once the straw is done, it needs to be drained well. Youll likely need to experiment here to see if you need to supplement with additional minerals. Recipes usually call for two parts manure with one part coco coir. Before starting my business I was also thinking about some things, then I ha. . 615 W State Street. It has already helped me so many times in understanding of cultivating mushrooms, Good luck nad God bless, Hey Andri! Growing on cardboard is so simple that you dont really need a recipe. If so, you may just be a little mystified which is totally understandable. Spawn rate. At this step, you've already prepared a liquid spore syringe. If you do choose to go the low tech route to grow you mushrooms, good substrate options include straw, straw pellets, sawdust pellets, sugar cane mulch, and coffee grounds. You also have the option to opt-out of these cookies. of dry substrate is considered to be 100% Biological Efficiency. Accessibility shouldnt be a problem for wood chips, since it is usually a by-product of other product making processes. And if you are regular visitors to the Garden Bench Top, you will know we are huge advocates of anything to do with reinventing waste into a functional purpose. General Cultivation; Getting Started . So youve decided you want to move beyond the pre-produced grow kits and follow the process of growing gourmet mushrooms from start to finish? Once you have sterilized you have surroundings it is time to inject the liquid culture into your chosen jar of sterilized spawn substrate. Note: The method described above is perfect for beginners because it ensures you wont contaminate the substrate if you keep investigating the substrate for growth which is a common trait for noobs (I used to check my jars hourly at one time, a little obsessive :D). Growing Mushrooms. A commonly discussed way to increase yield is to use a decent substrate such a master mix (Hardwood Sawdust + Soy Hulls + Water). High-nutrient substrates need to be sterilized to give the mushrooms youre growing a head start. One thing to watch out for is when mixing substrates, also known as supplementing them. But opting out of some of these cookies may affect your browsing experience. So we have decided to put together a neat little FAQ section that addresses a lot of queries that may crop up along your mushroom growing journey. Loc: Orbiting Earth. Enoki. You may be able to dispose of the used substrate in compost bins that get emptied each week. They will continue to grow for an additional 4-5 weeks with one "flush" or batch per week on average. Hey! How To Use A Pressure Cooker For Mushroom Cultivation. Similar to peat moss, perlite has great water absorption capabilities, which means mushrooms will thrive, especially during the fruiting stages. Oat straw. Types of substrate for mushroom growing: grain, bulk substrate, casing. As mushrooms increase in popularity, more people are getting into the business of fresh oyster mushroom cultivation. See our article How To Set Up A Low Tech Mushroom Farm for a full guide. It just requires additional labor and equipment. the different types of mushroom substrates, specific mushroom substrate recipes for edible mushroom varieties, and. They can be sauted, grilled, roasted, or even eaten raw. But once you supplement it with another material that makes it easier for mold or bacteria to grow, sterilization becomes necessary. One of the properties that is a must for mushrooms is access of water and humidity something peat moss excels at as it can hold large amounts of water content. So we need to do something to give mushrooms a bit of a head-start and ensure they colonize the substrate before other forms of mold or fungi can. If left to nature, these contaminants will outperform mycelium and take control of the substrate before mushrooms have a chance to get established in most cases. You dont want to use wood that has been dead or dying for a while already. Your lids should look like this. What do you do with Spent Mushroom Substrate? Water will need to be added to the substrate and it may also need to be amended with extra nutrients. Some home mushroom cultivators also like to mix different textured peat to encourage varying growth patterns in the mushroom growth cycle. For some species, I like to soak the substrate in water or give it a heavy misting to see if it fruits for a second time! But with our mushroom kit none of these worries exists. All you need to do is layer the mushroom between layers of damp cardboard and mycelium will start to grow. The strain you choose for your grow can have a huge impact on yield. You will typically see your first batch of mushrooms in the 4th or 5th week. The latter is preferably since its already pasteurized. Youll need to experiment to see how much supplementation to add, and the amount will also depend on whether the material youre adding is sterilized. After your mushrooms are done fruiting, you can dispose of spent substrate by composting it. If you sterilize your substrate this way, youll need to use distilled water to rehydrate it afterward while still keeping it sterile. One pound of oyster mushroom spawn is typically enough to inoculate 5-10 pounds of substrate. This is basically the same process but involving straw. We answer all the important questions when it comes to choosing the best substrate for growing mushrooms. A 10 lb bag can very likely give a higher yield- but it will depend on so many factors, and you just need to experiment to see what works. Emergency Info; Info For Parents; Link To Us; Search; Site Map; Sponsors; Mushroom Info. Hi, did I hear you say hay is used as a substrate You are an especially excellent experimenter and instructor. A few other species of mushrooms like shiitake may grow on coffee grounds as well, but might not produce as good of a harvest compared to growing on hardwood. Spawn rate is the amount of spawn used per kg on substrate on a dry weight basis. a 50/50 blend of soybean hulls and sawdust -- yields can be over 1 lb per 5 lb block on the first flush. . Because mushrooms enjoy a moist environment, a lot of spent mushroom substrate is great for spreading in and around your garden for water retention purposes. However when youre just learning how to make mushroom substrate, we recommend sticking with a single substrate to begin with until you can produce a consistent harvest. You dont want to be handling and heating up animal poop on a regular basis. Step 3: Innoculate your sterilized spawn with liquid culture. grow in a substrate composed of 100% straw, How To Grow Mushrooms On Logs: The Ultimate Guide, flushes of mushrooms from your compost pile, How To Grow Mushrooms: The Ultimate Guide, How To Grow Oyster Mushrooms: The Ultimate Step By Step Guide. . Prepare the substrate. While thats an ideal environment for mushrooms, its also the ideal conditions for other things like mold and bacteria to grow. You will be able to recognize if you have any intruders in your substrate with the appearance of mold.
The whole mixture then needs to be sterilized before it can be inoculated with mushroom spawn. Biological efficiency, often referred to as BE, is simply a way to calculate the effectiveness of a mushroom strain and substrate combination when growing mushrooms. Image The Kirks are 64 and . The main exceptions are button, crimini, and portobello mushrooms. On the flip-side, using more spawn per bulk substrate will decrease the chances of contamination; however, your mushroom yield will also be smaller. This kind of Low Tech Mushroom Farm may lead to slightly higher incidents of contamination though. This ratio will give you a 100% greater mushroom yield; however, it will also give you the greatest chance of contamination . That will enable you to branch out into many types of mushrooms. The shorter, more mature or dense the compost the less water it should have. Your substrate needs to be slightly acidic, with a PH level of about 5 6.5. Most commonly, supplement materials are bran or seed derivatives. I recommend one of these three ratios: 1:1 Spawn to Bulk Substrate. We find that as long as you follow basic procedures for sterilization or pasteurization of your mushroom substrates, the chance of contamination is very low. What Is a Mushroom Substrate? Again, too much spawn will eventually lead to diminishing returns. . Yields will be different depending on whether youre using a mss or a clone/ isolate and the size of the tub. Tthe Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to Amazon.com. The most important factor in whether you need to sterilize or pasteurize is the nutrition content of the substrate.
The whole mixture then needs to be sterilized before it can be inoculated with mushroom spawn. Biological efficiency, often referred to as BE, is simply a way to calculate the effectiveness of a mushroom strain and substrate combination when growing mushrooms. Image The Kirks are 64 and . The main exceptions are button, crimini, and portobello mushrooms. On the flip-side, using more spawn per bulk substrate will decrease the chances of contamination; however, your mushroom yield will also be smaller. This kind of Low Tech Mushroom Farm may lead to slightly higher incidents of contamination though. This ratio will give you a 100% greater mushroom yield; however, it will also give you the greatest chance of contamination . That will enable you to branch out into many types of mushrooms. The shorter, more mature or dense the compost the less water it should have. Your substrate needs to be slightly acidic, with a PH level of about 5 6.5. Most commonly, supplement materials are bran or seed derivatives. I recommend one of these three ratios: 1:1 Spawn to Bulk Substrate. We find that as long as you follow basic procedures for sterilization or pasteurization of your mushroom substrates, the chance of contamination is very low. What Is a Mushroom Substrate? Again, too much spawn will eventually lead to diminishing returns. . Yields will be different depending on whether youre using a mss or a clone/ isolate and the size of the tub. Tthe Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to Amazon.com. The most important factor in whether you need to sterilize or pasteurize is the nutrition content of the substrate.